Biological systems are genuinely illustrative of a complex macroscopic phenomenon being acutely controlled by microscopic chemical reaction networks. As a chemist’s dream to make an exact versatile and responsive material wraths on, research has intensified to create synthetic mimics of rudimentary biological processes. One such biological process is controlled development of cytoskeletal proteins. Aside from going about as nano skeleton to maintain cell shape, assemblies of these proteins are are responsible for distributing nutrients inside a cell. This results in them controlling almost every important process inside the cell, from division to constrain circulation. It is protected to assume that gathering control pathways of these proteins are a key giver in a phone’s versatile and responsive behaviour. The key piece of this procedure is an ATP fuelled metabolic framework that programs the rate of growth and decay of these assemblies in a time-related (temporal) manner. Close control is also maintained on the size of these assemblies as size directly relates to the functional efficacy of a system.
In an as of late published work in Nature Communications on Jan 25, 2019, scientists from the Jawaharlal Nehru Centre for Advanced Science and Research (JNCASR) and the Institute for Stem Cell Biology and Regenerative Medicine (inStem) have successfully created a minimalistic synthetic mimic of aforementioned cytoskeletal networks with structural and temporal programming.
The work centers around response driven controlled growth a two-component monomeric sub-atomic framework.
“This synthetic monomeric system is elegantly designed, so as to give rise to a primary reaction that would convert an inactive (non-assembling) monomeric system into an active one (which triggers assembly) upon addition of alkyl amines (fuel),” said Dr. Subi George, Associate Professor at JNCASR.
They demonstrate that this reaction can be utilized to exactly control the one-dimensional development (nanofibers) of coming about gatherings through controlled accessibility of “fuel”. The growth of these nanofibrous structures are driven by exceptionally powerless intermolecular interactions (termed as ‘supramolecular polymerization’ process) such as hydrophobic (similar to lipid bilayers in membranes) and aromatic interactions (similar to stacking of nucleobases in DNA) and hence are highly dynamic and have self-repairing features like many biological assemblies.
“While biological systems elegantly modulate self-assembly with a great precision, imparting transient and living polymerization property in chemical amphiphiles has been daunting task thus far. Design of an in-situ forming amphiphile enabled us to study the dynamic assembled structures at will,” said Dr. Praveen Kumar Vemula, Asst. Investigator at inStem.
Through itemized spectroscopic and microscopic analyses, they set up this development to be “living” in nature giving congregations with exceptionally thin size dispersion (monodisperse). The framework was additionally created by coupling the development with uniquely chosen chemical scenarios such that a unique control over the growth as well as the disassembly kinetics was established. As a result, a time-programmed transient network of fibrous assemblies could be realized. Thus, In both cases the manipulation of key temporal characteristics was from the range of few seconds to thousands of seconds. This study thus represents a key step in the development of adaptive, lifelike, supramolecular materials.
“We have for the first time demonstrated that every temporal characteristic of supramolecular polymerization can be chemically controlled and further coupled to other participating reactions similar to a biological system,” said Ankit Jain, lead author of the paper.
“Controlling the self-assembled nano-architectures using stimuli such as enzymes and pH has been fascinating,” says Ashish Dhayani, author of the paper.
“This work is a significant advancement to design bio-mimetic active systems operating under out of equilibrium conditions, with spatio-temporal programming compared to the majority of the synthetic passive systems reported so far, which work under thermodynamic equilibrium with only spatial complexity.” said Shikha Dhiman, co-author of the paper.
The following test is to build synthetic lifelike systems that can think, learn and adjust as the living creatures do. This examination is one such introductory advance but there is still a long run for scientists to be able to do what nature does. The team is optimistic to apply this principle and utilize the dynamic self-assembly nano-architectures in the biological systems.
This research was supported by the Department of Science and Technology, and Department of Biotechnology.